Affinity chromatography protein Purification: Estimate the molecular weights using Rf values of the three prominent bands you see in your thrombin digest lane.
Affinity chromatography protein Purification. 1) Estimate the molecular weights using Rf values of the three prominent bands you see in your thrombin digest lane. 2) You have been provided with two results of a MALDI-TOF analysis (pp. 102-103 above). a) Calculate the actual molecular weight of the three bands by doing a ratio calculation, based … Read more