Double strand DNA break : Explain how Cas9 detects and cleaves DNA targets, and (eukaryotic) cellular responses to the resultant double strand DNA break.

Double strand DNA break Explain how Cas9 detects and cleaves DNA targets, and (eukaryotic) cellular responses to the resultant double strand DNA break. The word limit is 2000 words ± 10% (does not include figure legends and references but does include the in-text citations).