Expression, Purification and Enzymology of a medically important Aldo-Keto Reductase (AKR1C3): explore the kinetic properties of an unknown ligand against the purified protein.
Expression, Purification and Enzymology of a medically important Aldo-Keto Reductase (AKR1C3) Purify an over-expressed recombinant protein (AKR1C3) from bacteria, determine its purity, complete initial, then more detailed kinetic characterisation of the protein purified. Explore the kinetic properties of an unknown ligand against the purified protein. Interpret and follow an extended set of lab protocols over … Read more