Describe how you would use Agro-infiltration of leaves of the plant Nicotiana benthamiana to produce a protein-based vaccine based on the SARS-CoV-2 spike protein?

Describe how you would use Agro-infiltration of leaves of the plant Nicotiana benthamiana to produce a protein-based vaccine based on the SARS-CoV-2 spike protein?

Give a description of the expression vector that you would design and construct.Use diagram(s) as appropriate to provide an overview of the expression clone construction process

Write a description of the process of expressing your Agrobacterium tumefaciens binary vector construct transiently in N. benthamiana leaves via Agro-infiltration.

a description of the subsequent purification of the recombinant protein.

Give a description of briefly the formulation and delivery of the final vaccine.

Write an overview of the timescale from clone construction through transient protein expression to purification and vaccine formulation/delivery.